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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
09/08/2013 |
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Data da última atualização: |
13/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BROD, F. C. A.; DINON, A. Z.; KOLLING, D. J.; FARIA, J. C.; ARISI, A. C. M. |
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Afiliação: |
FÁBIO C. A. BROD, UNIVERSIDADE FEDERAL DE SANTA CATARINA; ANDRÉIA Z. DINON, UNIVERSIDADE FEDERAL DE SANTA CATARINA; DEISE J. KOLLING, UNIVERSIDADE FEDERAL DE SANTA CATARINA; JOSIAS CORREA DE FARIA, CNPAF; ANA C. M. ARISI, UNIVERSIDADE FEDERAL DE SANTA CATARINA. |
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Título: |
Development of plasmid DNA reference material for the quantification of genetically modified common bean Embrapa 5.1. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Journal of Agricultural and Food Chemistry, Easton, v. 61, n. 20, p. 4921-4926, May 2013. |
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DOI: |
dx.doi.org/10.1021/jf400928k |
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Idioma: |
Inglês |
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Conteúdo: |
The genetically modified (GM) common bean Embrapa 5.1 was recently approved for commercialization. The reliable detection and quantification of GM organisms is strongly dependent on validated methods as well as calibration systems. This work presents the development of a calibrant plasmid for Embrapa 5.1 common bean detection. The reaction parameters were determined and compared for both the plasmid DNA (pDNA) and the genomic DNA (gDNA). PCR efficiencies for pDNA were 81% for the construction-specific assays and 76% for the taxon-specific assay, whereas for gDNA efficiencies were 94 and 93%, respectively. The limits of detection (LOD) in both qPCR assays were 102 and 103 copies of gDNA and pDNA per PCR reaction, respectively. This is sufficient to detect 0.067 and 0.67% of GM common bean in 100 ng of DNA, respectively, which is in agreement with detecting the 1% GM content required by the Brazilian legislation. |
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Thesagro: |
Biotecnologia; Engenharia genética; Feijão; Organismo transgênico; Plasmideo. |
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Thesaurus Nal: |
Beans; Genetically modified organisms; Plasmids; Transgenic plants. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
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024 7 $adx.doi.org/10.1021/jf400928k$2DOI
100 1 $aBROD, F. C. A.
245 $aDevelopment of plasmid DNA reference material for the quantification of genetically modified common bean Embrapa 5.1.$h[electronic resource]
260 $c2013
520 $aThe genetically modified (GM) common bean Embrapa 5.1 was recently approved for commercialization. The reliable detection and quantification of GM organisms is strongly dependent on validated methods as well as calibration systems. This work presents the development of a calibrant plasmid for Embrapa 5.1 common bean detection. The reaction parameters were determined and compared for both the plasmid DNA (pDNA) and the genomic DNA (gDNA). PCR efficiencies for pDNA were 81% for the construction-specific assays and 76% for the taxon-specific assay, whereas for gDNA efficiencies were 94 and 93%, respectively. The limits of detection (LOD) in both qPCR assays were 102 and 103 copies of gDNA and pDNA per PCR reaction, respectively. This is sufficient to detect 0.067 and 0.67% of GM common bean in 100 ng of DNA, respectively, which is in agreement with detecting the 1% GM content required by the Brazilian legislation.
650 $aBeans
650 $aGenetically modified organisms
650 $aPlasmids
650 $aTransgenic plants
650 $aBiotecnologia
650 $aEngenharia genética
650 $aFeijão
650 $aOrganismo transgênico
650 $aPlasmideo
700 1 $aDINON, A. Z.
700 1 $aKOLLING, D. J.
700 1 $aFARIA, J. C.
700 1 $aARISI, A. C. M.
773 $tJournal of Agricultural and Food Chemistry, Easton$gv. 61, n. 20, p. 4921-4926, May 2013.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
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URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
16/06/2015 |
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Data da última atualização: |
11/03/2019 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
HOLCMAN, E.; SENTELHAS, P. C.; CONCEIÇÃO, M. A. F.; SPOSITO, M. B.; COUTO, H. T. Z. |
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Afiliação: |
MARCO ANTONIO FONSECA CONCEICAO, CNPUV. |
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Título: |
Downy mildew warning systems for vineyards cultivated under plastic covering - influence on yield and quality |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
In: International Congress of Biometeorology,20., 2014. American Meteorological Society, n. 22,sept. 2014. |
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Idioma: |
Português |
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Notas: |
Poster |
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Palavras-Chave: |
Doenças; Vineyards cultivated; Vinhas cultivadas. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/125428/1/CONCEICAO-20International-Congress-of-Biometeorology-2014.pdf
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Marc: |
LEADER 00681nam a2200193 a 4500
001 2017727
005 2019-03-11
008 2014 bl uuuu u00u1 u #d
100 1 $aHOLCMAN, E.
245 $aDowny mildew warning systems for vineyards cultivated under plastic covering - influence on yield and quality$h[electronic resource]
260 $aIn: International Congress of Biometeorology,20., 2014. American Meteorological Society, n. 22,sept. 2014.$c2014
500 $aPoster
653 $aDoenças
653 $aVineyards cultivated
653 $aVinhas cultivadas
700 1 $aSENTELHAS, P. C.
700 1 $aCONCEIÇÃO, M. A. F.
700 1 $aSPOSITO, M. B.
700 1 $aCOUTO, H. T. Z.
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Embrapa Uva e Vinho (CNPUV) |
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